細胞名稱

人肝癌細胞HuH-7

貨物編碼

BFN60807349

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研           1類

培養體系

DMEM高糖培養基+10%FBS+1%雙抗 

培養溫度

37℃

二氧化碳濃度

5%

簡介

人肝癌細胞HuH-7取自57歲男性供體，該細胞源于ATCC

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 3.

Part of: JFCR45 cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: TCGA-110-CL cell line panel.

Doubling time: ~48 hours (CLS).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突變

Homozygous for KDR p.Gln472His (c.1416A>T) (PubMed=29774518).

POLD3 p.Lys70Arg (c.209A>G) (DOI=10.1101/2020.02.17.953281).

Homozygous for TP53 p.Tyr220Cys (c.659A>G) (PubMed=8389256; PubMed=29774518).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        11

D1S1656        16

D2S1338        19

D3S1358        15

D5S818        12

D6S1043        13,15

D7S820        11

D8S1179        14,15

D12S391        20 (CLS)

20,21 (CCRID)

D13S317        10 (CLS; RCB)

10,11 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; TKG)

D16S539        10

D18S51        15

D19S433        13,14

D21S11        30

FGA        22,23

Penta D        12

Penta E        11

TH01        7

TPOX        8,11

vWA        16,18 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; RCB; TKG)

18 (CLS)

參考文獻

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

DOI=10.1101/2020.02.17.953281

Kawamoto M., Yamaji T., Saito K., Satomura K., Endo T., Fukasawa M., Hanada K., Osada N.

Identification of characteristic genomic markers in human hepatoma Huh7 and Huh7.5.1-8 cell lines.

bioRxiv 2020:953281-953281(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)