細(xì)胞名稱

人肝永生化細(xì)胞THLE-2

貨物編碼

BFN60808733

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研     2類

培養(yǎng)體系

DMEM高糖+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人肝永生化細(xì)胞THLE-2取自男性供體，經(jīng)過SV40永生化處理。該細(xì)胞源于ATCC

注釋

倍增時(shí)間33h

基因突變

/

HLA信息

STR信息

Amelogenin        X,Y

CSF1PO        11,13

D5S818        11,13

D7S820        10,12

D13S317        8,12

D16S539        11,13

TH01        7,9.3

TPOX        8,11

vWA        16,17

參考文獻(xiàn)

PubMed=7685115; DOI=10.1073/pnas.90.11.5123

Pfeifer A.M.A., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.M., Juillerat M., Lipsky M.M., Trump B.F., Lechner J.F., Harris C.C.

Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.

Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

Patent=US5759765

Harris C.C., Cole K.H., Lechner J.F., Reddel R.R.

Human liver epithelial cell lines.

Patent number US5759765, 02-Jun-1998

PubMed=23887712; DOI=10.1038/ncomms3218

Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.

High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.

Nat. Commun. 4:2218-2218(2013)