細(xì)胞名稱

人急性髓系白血病細(xì)胞Kasumi-6

貨物編碼

BFN60810164

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研   2類

培養(yǎng)體系

90%DMEM+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人急性髓系白血病細(xì)胞Kasumi-6取自64歲男性外周血，該細(xì)胞源于DSMZ

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: TCGA-110-CL cell line panel.

Population: Japanese and Korean.

Doubling time: ~70 hours (DSMZ).

Omics: Deep exome analysis.

Omics: Deep RNAseq analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突變

Heterozygous for CEBPA p.Arg306Pro (c.917G>C) (PubMed=12508245; CCLE).

FLT3 internal tandem duplication (FLT3-ITD) (PubMed=32180206).

Homozygous for TP53 p.Tyr234Asp (c.700T>G) (CCLE).

HLA信息

/

STR信息

Amelogenin        X,Y

CSF1PO        10,12

D5S818        11

D7S820        9,11

D13S317        8,12

D16S539        9,10

TH01        6,9

TPOX        8,9

vWA        17

參考文獻(xiàn)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

PubMed=32180206; DOI=10.1007/s13577-020-00347-5

Kasai F., Asou H., Ozawa M., Kobayashi K., Kuramitsu H., Satoh M., Kohara A., Kaneko Y., Kawamura M.

Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.

Hum. Cell 33:0-0(2020)